Journal: British Journal of Cancer

Article Title: Loss of Coxsackie and adenovirus receptor downregulates α -catenin expression

doi: 10.1038/sj.bjc.6605331

Figure Lengend Snippet: Impact of CAR and α -catenin on cellular morphology. Cells after CAR knockdown appear round and smaller compared with controls (DLD1) or display less intercellular attachment sites (IEC-6). In matrigel, cells after CAR knockdown form amorphous clusters in contrast to the organised-appearing formations of matching controls. Ectopic ‘re’-expression of α -catenin partially reverses the effects seen after CAR knockdown when grown as a monolayer, and particularly results in organised cell formations similar to those of vector controls when cultured in matrigel. Images show representative results for DLD1 ( A ) and IEC-6 ( B ).

Article Snippet: The human colon cancer cell line DLD1 and the rat small intestine cell line IEC-6 were obtained from the American Type Culture Collection (Rockville, MD, USA) and from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany), respectively, and were cultured in recommended growth media.

Techniques: Knockdown, Expressing, Plasmid Preparation, Cell Culture

Journal: British Journal of Cancer

Article Title: Loss of Coxsackie and adenovirus receptor downregulates α -catenin expression

doi: 10.1038/sj.bjc.6605331

Figure Lengend Snippet: Functional impact of CAR downregulation and α -catenin ‘re’-expression in DLD1 and IEC-6 cell lines. Proliferation ( A ), migration ( B ), and invasion ( C ) in DLD1 and IEC-6 were determined after CAR knockdown and ‘re’-expression of α -catenin in comparison with controls.

Article Snippet: The human colon cancer cell line DLD1 and the rat small intestine cell line IEC-6 were obtained from the American Type Culture Collection (Rockville, MD, USA) and from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany), respectively, and were cultured in recommended growth media.

Techniques: Functional Assay, Expressing, Migration, Knockdown, Comparison

Journal: Journal of Cellular and Molecular Medicine

Article Title: DPP7 Promotes Colorectal Cancer Progression Through GPX4 ‐Dependent Suppression of Disulfidptosis and Immune Evasion

doi: 10.1111/jcmm.70660

Figure Lengend Snippet: DPP7 is overexpressed in colorectal cancer and promotes tumour growth. (A) Kaplan–Meier overall survival analysis of TCGA‐COAD patients stratified by DPP7 expression levels ( n = 227 in each group, p = 0.0001). (B) Kaplan–Meier progression‐free survival analysis of TCGA‐COAD patients stratified by DPP7 expression levels ( n = 227 in each group, p = 0.0011). (C) DPP7 mRNA expression in tumour ( n = 455) versus normal ( n = 820) tissues from TCGA‐COAD dataset. (D) Relative DPP7 mRNA expression in various colorectal cancer cell lines compared to normal colon epithelial cell line (NCM). (E) Relative DPP7 mRNA expression in HCT116 and SW480 cells transfected with control siRNA (si‐NC) or DPP7‐specific siRNAs (si‐DPP7#1 and si‐DPP7#2). (F) Western blot analysis of DPP7 protein expression in HCT116 and SW480 cells transfected with indicated siRNAs. (G‐H) Cell growth curves of HCT116 (G and SW480 (H) cells transfected with indicated siRNAs measured by CCK‐8 assay. (I) Representative images of colony formation assay for HCT116 and SW480 cells transfected with indicated siRNAs. (J) Quantification of colony numbers from (I). (K) Flow cytometry analysis of apoptosis in HCT116 and SW480 cells transfected with indicated siRNAs. (L) Quantification of apoptotic cells from (K). (M) Western blot analysis of proliferation marker (PCNA) and apoptosis markers (Bcl‐2, Bax) in HCT116 and SW480 cells transfected with indicated siRNAs. GAPDH was used as a loading control. Data are presented as mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human colorectal cancer cell lines (HCT116, SW480, RKO, HC29, DLD‐1, HT29 and SW620) and the normal colon epithelial cell line (NCM460) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Transfection, Control, Western Blot, CCK-8 Assay, Colony Assay, Flow Cytometry, Marker